Requirements for Animal Cell Culture:
In the Table Sterilization by dry heat: Sterilization by moist heat: For effective moist heat sterilization, it is necessary that the steam penetrates to all the parts of the sterilizing materials.
The use of filters for sterilization of liquids often becomes necessary, since the constituents of these liquids may get destroyed at higher temperatures dry heat or moist heat. Sterile filtration is a novel technique for heat- labile solutions.
The size of micropores of the filters is 0. Filters, made from several materials are in use. These materials include nylon, cellulose acetate, cellulose nitrate, polycarbonate, polyethersulfone PES and ceramics.
The filters are made in different designs-disc filters, cartridges and hollow fiber. In fact, many commercial companies e.
Millipore, Durapore supply reusable and disposable filters, designed for different purposes of sterilization.
Advantages and Limitations of Tissue Culture: Advantages of Tissue Culture: Tissue culture technique has a wide range of applications.
The most important advantages of this technique are listed below: Control of physicochemical environment- pH, temperature, dissolved gases O2 and CO2osmolarity. Regulation of physiological conditions-nutrient concentration, cell to cell interactions, hormonal control.
The cultured cell lines become homogenous i. This is in contrast to the heterogenous cells of tissue samples. The homogenous cells are highly useful for a wide range of purposes.
It is easy to characterize cells for cytological and immunological studies.
Cultured cells can be stored in liquid nitrogen for several years.The Advances in Cell and Tissue Culture international conference aims to create an interdisciplinary forum in which over industry and academic researchers, working in cell culture can exchange knowledge, promote their activities and set up new collaborative projects.
Tissue culture: Tissue culture, a method of biological research in which fragments of tissue from an animal or plant are transferred to an artificial environment in which they can continue to survive and function. The cultured tissue may consist of a single cell, a population of cells, or a whole or part of an.
"Advantage And Disadvantage Of Animal Cell Tissue Culture" Essays and Research Papers. Advantage And Disadvantage Of Animal Cell Tissue Culture. primary tissue culture Now-a -days it has become possible to use the cell and tissue culture in the areas of research which have a potential for economic value and commercialization.
Primary animal tissue and micro-organisms must not be cultured in or near the cell culture laboratory and the laboratory must be specifically designated for clean cell culture work.
b. Incubation Facilities. Essay on Animal Tissue Culture Essay # 1. Introduction to Animal Tissue Culture: The term tissue culture refers to the culture of whole organs, tissue fragments as well as dispersed cells on a suitable nutrient medium.
Essay on Animal Tissue Culture (High Quality Essays) | Zoology.
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